Quantitative Biuret Determination of Urine Protein
نویسندگان
چکیده
منابع مشابه
Determination of protein in urine by the biuret method.
A method for the determination of protein in urine by means of the biuret reaction was described by Hiller (1) and by Hiller, McIntosh, and Van Slyke (2), in which pure biuret prepared by Kahlbaum was used as a standard for visual calorimetry. Since Hiller (1) the biuret method has been used for the determination of urinary proteins by Price (3) and by Lehmann (4), and has been widely used in m...
متن کاملA biuret method for determination of protein in normal urine.
A biuret method has been developed which provides quantitative measurements of protein in normal urine without interference from drugs or pigments. This method is intended for use in monitoring clinical trials of new drugs-to detect nephrotoxicity. Protein is precipitated from duplicate samples of urine by addition of cold ethanolic phosphotungstic acid. The protein precipitates are separated b...
متن کاملDetermination of Protein in Urine by the Biuret Method
A method for the determination of protein in urine by means of the biuret reaction was described by Hiller (1) and by Hiller, McIntosh, and Van Slyke (2), in which pure biuret prepared by Kahlbaum was used as a standard for visual calorimetry. Since Hiller (1) the biuret method has been used for the determination of urinary proteins by Price (3) and by Lehmann (4), and has been widely used in m...
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Total urinary protein is rapidly precipitated at room temperature by tannic acid. The tannic acid/protein precipitate, dissolved in aqueous triethanolamine/ferric chloride solution, gives a purple-violet color of high absorptivity. Absorbance at 510 nm is linearly related to concentration from 0.05 to 1.50 A for a protein content of 0.05 to 1.50 g/liter, and less than 5 mg/liter can be detected...
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HE INTRODUCTION OF the carboxylic acid cation exchange Amberlite XE-96 (or IRC-50) Azure A resin’ as the medium for hydrogen ion exchange in place of the quininium exchange resin has simplified greatly the determination of gastric acidity without intubation (1). Solvent extraction and fluorimetry are no longer required. The amount of blue dye excreted in the urine is readily measured by visual ...
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ژورنال
عنوان ژورنال: Proceedings of the Association of Clinical Biochemists
سال: 1968
ISSN: 0369-8564
DOI: 10.1177/036985646800500315